Rhizosphere microbiomes diverge among Populus trichocarpa plant-host genotypes and chemotypes, but it depends on soil origin.
Identifieur interne : 000727 ( Main/Exploration ); précédent : 000726; suivant : 000728Rhizosphere microbiomes diverge among Populus trichocarpa plant-host genotypes and chemotypes, but it depends on soil origin.
Auteurs : Allison M. Veach [États-Unis] ; Reese Morris [États-Unis] ; Daniel Z. Yip [États-Unis] ; Zamin K. Yang [États-Unis] ; Nancy L. Engle [États-Unis] ; Melissa A. Cregger [États-Unis] ; Timothy J. Tschaplinski [États-Unis] ; Christopher W. Schadt [États-Unis]Source :
- Microbiome [ 2049-2618 ] ; 2019.
Descripteurs français
- KwdFr :
- ARN ribosomique 16S (génétique), Acide salicylique (métabolisme), Analyse de séquence d'ADN (MeSH), Archéobactéries (classification), Bactéries (classification), Champignons (classification), Génotype (MeSH), Microbiologie du sol (MeSH), Microbiote (MeSH), Métabolisme secondaire (MeSH), Métabolomique (MeSH), Populus (génétique), Populus (microbiologie), Populus (métabolisme), Racines de plante (microbiologie), Rhizosphère (MeSH).
- MESH :
- génétique : ARN ribosomique 16S, Archéobactéries, Bactéries, Champignons, Populus.
- microbiologie : Populus, Racines de plante.
- métabolisme : Acide salicylique, Populus.
- Analyse de séquence d'ADN, Génotype, Microbiologie du sol, Microbiote, Métabolisme secondaire, Métabolomique, Rhizosphère.
English descriptors
- KwdEn :
- Archaea (classification), Bacteria (classification), Fungi (classification), Genotype (MeSH), Metabolomics (MeSH), Microbiota (MeSH), Plant Roots (microbiology), Populus (genetics), Populus (metabolism), Populus (microbiology), RNA, Ribosomal, 16S (genetics), Rhizosphere (MeSH), Salicylic Acid (metabolism), Secondary Metabolism (MeSH), Sequence Analysis, DNA (MeSH), Soil Microbiology (MeSH).
- MESH :
- chemical , genetics : RNA, Ribosomal, 16S.
- classification : Archaea, Bacteria, Fungi.
- genetics : Populus.
- metabolism : Populus, Salicylic Acid.
- microbiology : Plant Roots, Populus.
- Genotype, Metabolomics, Microbiota, Rhizosphere, Secondary Metabolism, Sequence Analysis, DNA, Soil Microbiology.
Abstract
BACKGROUND
Plants have developed defense strategies for phytopathogen and herbivore protection via coordinated metabolic mechanisms. Low-molecular weight metabolites produced within plant tissues, such as salicylic acid, represent one such mechanism which likely mediates plant - microbe interactions above and below ground. Salicylic acid is a ubiquitous phytohormone at low levels in most plants, yet are concentrated defense compounds in Populus, likely acting as a selective filter for rhizosphere microbiomes. We propagated twelve Populus trichocarpa genotypes which varied an order of magnitude in salicylic acid (SA)-related secondary metabolites, in contrasting soils from two different origins. After four months of growth, plant properties (leaf growth, chlorophyll content, and net photosynthetic rate) and plant root metabolomics specifically targeting SA metabolites were measured via GC-MS. In addition, rhizosphere microbiome composition was measured via Illumina MiSeq sequencing of 16S and ITS2 rRNA-genes.
RESULTS
Soil origin was the primary filter causing divergence in bacterial/archaeal and fungal communities with plant genotype secondarily influential. Both bacterial/archaeal and fungal evenness varied between soil origins and bacterial/archaeal diversity and evenness correlated with at least one SA metabolite (diversity: populin; evenness: total phenolics). The production of individual salicylic acid derivatives that varied by host genotype resulted in compositional differences for bacteria /archaea (tremuloidin) and fungi (salicylic acid) within one soil origin (Clatskanie) whereas soils from Corvallis did not illicit microbial compositional changes due to salicylic acid derivatives. Several dominant bacterial (e.g., Betaproteobacteria, Acidobacteria, Verrucomicrobia, Chloroflexi, Gemmatimonadete, Firmicutes) and one fungal phyla (Mortierellomycota) also correlated with specific SA secondary metabolites; bacterial phyla exhibited more negative interactions (declining abundance with increasing metabolite concentration) than positive interactions.
CONCLUSIONS
These results indicate microbial communities diverge most among soil origin. However, within a soil origin, bacterial/archaeal communities are responsive to plant SA production within greenhouse-based rhizosphere microbiomes. Fungal microbiomes are impacted by root SA-metabolites, but overall to a lesser degree within this experimental context. These results suggest plant defense strategies, such as SA and its secondary metabolites, may partially drive patterns of both bacterial/archaeal and fungal taxa-specific colonization and assembly.
DOI: 10.1186/s40168-019-0668-8
PubMed: 31103040
PubMed Central: PMC6525979
Affiliations:
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Tschaplinski</name><affiliation wicri:level="1"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038</wicri:regionArea><wicri:noRegion>37831-6038</wicri:noRegion></affiliation></author><author><name sortKey="Schadt, Christopher W" sort="Schadt, Christopher W" uniqKey="Schadt C" first="Christopher W" last="Schadt">Christopher W. Schadt</name><affiliation wicri:level="1"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038, USA. schadtcw@ornl.gov.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038</wicri:regionArea><wicri:noRegion>37831-6038</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Tennessee, Knoxville, TN, 37996, USA. schadtcw@ornl.gov.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology, University of Tennessee, Knoxville, TN, 37996</wicri:regionArea><wicri:noRegion>37996</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31103040</idno><idno type="pmid">31103040</idno><idno type="doi">10.1186/s40168-019-0668-8</idno><idno type="pmc">PMC6525979</idno><idno type="wicri:Area/Main/Corpus">000899</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000899</idno><idno type="wicri:Area/Main/Curation">000899</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000899</idno><idno type="wicri:Area/Main/Exploration">000899</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Rhizosphere microbiomes diverge among Populus trichocarpa plant-host genotypes and chemotypes, but it depends on soil origin.</title><author><name sortKey="Veach, Allison M" sort="Veach, Allison M" uniqKey="Veach A" first="Allison M" last="Veach">Allison M. Veach</name><affiliation wicri:level="1"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038</wicri:regionArea><wicri:noRegion>37831-6038</wicri:noRegion></affiliation></author><author><name sortKey="Morris, Reese" sort="Morris, Reese" uniqKey="Morris R" first="Reese" last="Morris">Reese Morris</name><affiliation wicri:level="1"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038</wicri:regionArea><wicri:noRegion>37831-6038</wicri:noRegion></affiliation></author><author><name sortKey="Yip, Daniel Z" sort="Yip, Daniel Z" uniqKey="Yip D" first="Daniel Z" last="Yip">Daniel Z. Yip</name><affiliation wicri:level="1"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038</wicri:regionArea><wicri:noRegion>37831-6038</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Zamin K" sort="Yang, Zamin K" uniqKey="Yang Z" first="Zamin K" last="Yang">Zamin K. Yang</name><affiliation wicri:level="1"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038</wicri:regionArea><wicri:noRegion>37831-6038</wicri:noRegion></affiliation></author><author><name sortKey="Engle, Nancy L" sort="Engle, Nancy L" uniqKey="Engle N" first="Nancy L" last="Engle">Nancy L. Engle</name><affiliation wicri:level="1"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038</wicri:regionArea><wicri:noRegion>37831-6038</wicri:noRegion></affiliation></author><author><name sortKey="Cregger, Melissa A" sort="Cregger, Melissa A" uniqKey="Cregger M" first="Melissa A" last="Cregger">Melissa A. Cregger</name><affiliation wicri:level="1"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038</wicri:regionArea><wicri:noRegion>37831-6038</wicri:noRegion></affiliation></author><author><name sortKey="Tschaplinski, Timothy J" sort="Tschaplinski, Timothy J" uniqKey="Tschaplinski T" first="Timothy J" last="Tschaplinski">Timothy J. Tschaplinski</name><affiliation wicri:level="1"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038</wicri:regionArea><wicri:noRegion>37831-6038</wicri:noRegion></affiliation></author><author><name sortKey="Schadt, Christopher W" sort="Schadt, Christopher W" uniqKey="Schadt C" first="Christopher W" last="Schadt">Christopher W. Schadt</name><affiliation wicri:level="1"><nlm:affiliation>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038, USA. schadtcw@ornl.gov.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038</wicri:regionArea><wicri:noRegion>37831-6038</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, University of Tennessee, Knoxville, TN, 37996, USA. schadtcw@ornl.gov.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology, University of Tennessee, Knoxville, TN, 37996</wicri:regionArea><wicri:noRegion>37996</wicri:noRegion></affiliation></author></analytic><series><title level="j">Microbiome</title><idno type="eISSN">2049-2618</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Archaea (classification)</term><term>Bacteria (classification)</term><term>Fungi (classification)</term><term>Genotype (MeSH)</term><term>Metabolomics (MeSH)</term><term>Microbiota (MeSH)</term><term>Plant Roots (microbiology)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Populus (microbiology)</term><term>RNA, Ribosomal, 16S (genetics)</term><term>Rhizosphere (MeSH)</term><term>Salicylic Acid (metabolism)</term><term>Secondary Metabolism (MeSH)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Soil Microbiology (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN ribosomique 16S (génétique)</term><term>Acide salicylique (métabolisme)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Archéobactéries (classification)</term><term>Bactéries (classification)</term><term>Champignons (classification)</term><term>Génotype (MeSH)</term><term>Microbiologie du sol (MeSH)</term><term>Microbiote (MeSH)</term><term>Métabolisme secondaire (MeSH)</term><term>Métabolomique (MeSH)</term><term>Populus (génétique)</term><term>Populus (microbiologie)</term><term>Populus (métabolisme)</term><term>Racines de plante (microbiologie)</term><term>Rhizosphère (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Ribosomal, 16S</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Archaea</term><term>Bacteria</term><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN ribosomique 16S</term><term>Archéobactéries</term><term>Bactéries</term><term>Champignons</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term><term>Salicylic Acid</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Populus</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Roots</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acide salicylique</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genotype</term><term>Metabolomics</term><term>Microbiota</term><term>Rhizosphere</term><term>Secondary Metabolism</term><term>Sequence Analysis, DNA</term><term>Soil Microbiology</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Génotype</term><term>Microbiologie du sol</term><term>Microbiote</term><term>Métabolisme secondaire</term><term>Métabolomique</term><term>Rhizosphère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Plants have developed defense strategies for phytopathogen and herbivore protection via coordinated metabolic mechanisms. Low-molecular weight metabolites produced within plant tissues, such as salicylic acid, represent one such mechanism which likely mediates plant - microbe interactions above and below ground. Salicylic acid is a ubiquitous phytohormone at low levels in most plants, yet are concentrated defense compounds in Populus, likely acting as a selective filter for rhizosphere microbiomes. We propagated twelve Populus trichocarpa genotypes which varied an order of magnitude in salicylic acid (SA)-related secondary metabolites, in contrasting soils from two different origins. After four months of growth, plant properties (leaf growth, chlorophyll content, and net photosynthetic rate) and plant root metabolomics specifically targeting SA metabolites were measured via GC-MS. In addition, rhizosphere microbiome composition was measured via Illumina MiSeq sequencing of 16S and ITS2 rRNA-genes.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Soil origin was the primary filter causing divergence in bacterial/archaeal and fungal communities with plant genotype secondarily influential. Both bacterial/archaeal and fungal evenness varied between soil origins and bacterial/archaeal diversity and evenness correlated with at least one SA metabolite (diversity: populin; evenness: total phenolics). The production of individual salicylic acid derivatives that varied by host genotype resulted in compositional differences for bacteria /archaea (tremuloidin) and fungi (salicylic acid) within one soil origin (Clatskanie) whereas soils from Corvallis did not illicit microbial compositional changes due to salicylic acid derivatives. Several dominant bacterial (e.g., Betaproteobacteria, Acidobacteria, Verrucomicrobia, Chloroflexi, Gemmatimonadete, Firmicutes) and one fungal phyla (Mortierellomycota) also correlated with specific SA secondary metabolites; bacterial phyla exhibited more negative interactions (declining abundance with increasing metabolite concentration) than positive interactions.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>These results indicate microbial communities diverge most among soil origin. However, within a soil origin, bacterial/archaeal communities are responsive to plant SA production within greenhouse-based rhizosphere microbiomes. Fungal microbiomes are impacted by root SA-metabolites, but overall to a lesser degree within this experimental context. These results suggest plant defense strategies, such as SA and its secondary metabolites, may partially drive patterns of both bacterial/archaeal and fungal taxa-specific colonization and assembly.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31103040</PMID><DateCompleted><Year>2020</Year><Month>04</Month><Day>30</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>30</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2049-2618</ISSN><JournalIssue CitedMedium="Internet"><Volume>7</Volume><Issue>1</Issue><PubDate><Year>2019</Year><Month>05</Month><Day>18</Day></PubDate></JournalIssue><Title>Microbiome</Title><ISOAbbreviation>Microbiome</ISOAbbreviation></Journal><ArticleTitle>Rhizosphere microbiomes diverge among Populus trichocarpa plant-host genotypes and chemotypes, but it depends on soil origin.</ArticleTitle><Pagination><MedlinePgn>76</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s40168-019-0668-8</ELocationID><Abstract><AbstractText Label="BACKGROUND">Plants have developed defense strategies for phytopathogen and herbivore protection via coordinated metabolic mechanisms. Low-molecular weight metabolites produced within plant tissues, such as salicylic acid, represent one such mechanism which likely mediates plant - microbe interactions above and below ground. Salicylic acid is a ubiquitous phytohormone at low levels in most plants, yet are concentrated defense compounds in Populus, likely acting as a selective filter for rhizosphere microbiomes. We propagated twelve Populus trichocarpa genotypes which varied an order of magnitude in salicylic acid (SA)-related secondary metabolites, in contrasting soils from two different origins. After four months of growth, plant properties (leaf growth, chlorophyll content, and net photosynthetic rate) and plant root metabolomics specifically targeting SA metabolites were measured via GC-MS. In addition, rhizosphere microbiome composition was measured via Illumina MiSeq sequencing of 16S and ITS2 rRNA-genes.</AbstractText><AbstractText Label="RESULTS">Soil origin was the primary filter causing divergence in bacterial/archaeal and fungal communities with plant genotype secondarily influential. Both bacterial/archaeal and fungal evenness varied between soil origins and bacterial/archaeal diversity and evenness correlated with at least one SA metabolite (diversity: populin; evenness: total phenolics). The production of individual salicylic acid derivatives that varied by host genotype resulted in compositional differences for bacteria /archaea (tremuloidin) and fungi (salicylic acid) within one soil origin (Clatskanie) whereas soils from Corvallis did not illicit microbial compositional changes due to salicylic acid derivatives. Several dominant bacterial (e.g., Betaproteobacteria, Acidobacteria, Verrucomicrobia, Chloroflexi, Gemmatimonadete, Firmicutes) and one fungal phyla (Mortierellomycota) also correlated with specific SA secondary metabolites; bacterial phyla exhibited more negative interactions (declining abundance with increasing metabolite concentration) than positive interactions.</AbstractText><AbstractText Label="CONCLUSIONS">These results indicate microbial communities diverge most among soil origin. However, within a soil origin, bacterial/archaeal communities are responsive to plant SA production within greenhouse-based rhizosphere microbiomes. Fungal microbiomes are impacted by root SA-metabolites, but overall to a lesser degree within this experimental context. These results suggest plant defense strategies, such as SA and its secondary metabolites, may partially drive patterns of both bacterial/archaeal and fungal taxa-specific colonization and assembly.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Veach</LastName><ForeName>Allison M</ForeName><Initials>AM</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Morris</LastName><ForeName>Reese</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yip</LastName><ForeName>Daniel Z</ForeName><Initials>DZ</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Zamin K</ForeName><Initials>ZK</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Engle</LastName><ForeName>Nancy L</ForeName><Initials>NL</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cregger</LastName><ForeName>Melissa A</ForeName><Initials>MA</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tschaplinski</LastName><ForeName>Timothy J</ForeName><Initials>TJ</Initials><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schadt</LastName><ForeName>Christopher W</ForeName><Initials>CW</Initials><Identifier Source="ORCID">0000-0001-8759-2448</Identifier><AffiliationInfo><Affiliation>Biosciences Division, Oak Ridge National Laboratory, 1 Bethel Valley Rd, Oak Ridge, TN, 37831-6038, USA. schadtcw@ornl.gov.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Microbiology, University of Tennessee, Knoxville, TN, 37996, USA. schadtcw@ornl.gov.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>05</Month><Day>18</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Microbiome</MedlineTA><NlmUniqueID>101615147</NlmUniqueID><ISSNLinking>2049-2618</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012336">RNA, Ribosomal, 16S</NameOfSubstance></Chemical><Chemical><RegistryNumber>O414PZ4LPZ</RegistryNumber><NameOfSubstance UI="D020156">Salicylic Acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001105" MajorTopicYN="N">Archaea</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001419" MajorTopicYN="N">Bacteria</DescriptorName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005658" 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16S</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D058441" MajorTopicYN="Y">Rhizosphere</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020156" MajorTopicYN="N">Salicylic Acid</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D064210" MajorTopicYN="N">Secondary Metabolism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017422" MajorTopicYN="N">Sequence Analysis, DNA</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012988" MajorTopicYN="Y">Soil Microbiology</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">16S rRNA</Keyword><Keyword MajorTopicYN="Y">ITS2</Keyword><Keyword MajorTopicYN="Y">Metabolomics</Keyword><Keyword MajorTopicYN="Y">Populus trichocarpa</Keyword><Keyword MajorTopicYN="Y">Rhizosphere</Keyword><Keyword 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